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Introduction
Methodology
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BLAST
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Organism:
Jatropha curcas
Tissue:
Roots
cDNA library:
Created in pYES2.1 TOPO TA cloning vector using Super SMART cDNA synthesis kit.
Not directionally inserted.
ESTs:
Around 60% sequences generated with forward primer (Gal 1) and 40% sequences
generated with reverse primer (V/5 C-term).
Total number of Raw ESTs:
1231
Total number of unigenes set:
866
Number of singletons:
758
Number of Contigs:
108
Full length:
69
Source: Plant Metabolic Engineering Group, Reliance Life Sciences, Dhirubhai Ambani Life Science Center, , Rabale, Navi Mumbai, India
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